Analysis Note

Positive ControlHepG2, NIH 3T3 cells, MCF-7 cells, colon or breast adenocarcinoma tissue

Application

Frozen sections (5-10 µg/ml)r>Immunoblotting (1-5 µg/ml)r>Immunofluorescence (1-5 µg/ml)r>Immunoprecipitation (1-2 µg/reaction)r>Paraffin sections (5-10 µg/ml, pepsin or heat pre-treatment required)r>Free-floating Sections (not recommended)

General description

This Anti-c-Fos (Ab-2) (4-17) Rabbit pAb is validated for use in Frozen sections, Immunoblotting, IF, IP, Paraffin sections, for the detection of c-Fos (Ab-2) (4-17).

Protein A purified rabbit polyclonal antibody. Recognizes v-fos and ~50-62 kDa c-fos proteins.

The proto-oncogene fos has been implicated in cell growth, differentiation and development. Fos is induced by a large number of stimuli including mitogens, differentiation-specific agents, pharmacological agents, etc. Induction of the 62,000 dalton fos protein is rapid but transient. The fos protein is associated with a 39,000 dalton protein which has been identified as the protein product of the jun proto-oncogene (c-jun). The fos/jun protein complex binds specifically to a sequence element in DNA referred to as the AP-1 binding site.

Recognizes the ~50-62 kDa c-Fos protein in HepG2, NIH3T3, and MCF-7 cells.

Immunogen

a synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For gel shift assays, use Cat. No. PC05L resuspended in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibody should be titrated for optimal results in individual systems.

DeTogni, P., et al. 1988. Cell Biol.8, 2251.Rouscher III, F.J., et al. 1988. Science240, 1010.Sassone-Corsi, P., et al. 1988. Cell54, 553.Verma, I.M. and Graham, W.R. 1987. Cancer Res.49, 29.Verma, I.M. and Sassone-Corsi, P. 1987. Cell51, 513.Muller, R. 1986. Biochim. Biophys. Acta823, 207.Verma, I. 1986. Trends Genet.2, 93.Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433.

Packaging

100 µg in Glass bottle

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Warning

Toxicity: Highly Toxic (H)